Agrobacterium rhizogenes Transformation of the Phaseolus spp.: A Tool for Functional Genomics
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چکیده
منابع مشابه
Agrobacterium rhizogenes-Mediated Transformation of the Parasitic Plant Phtheirospermum japonicum
BACKGROUND Plants within the Orobanchaceae are an agriculturally important group of parasites that attack economically important crops to obtain water and nutrients from their hosts. Despite their agricultural importance, molecular mechanisms of the parasitism are poorly understood. METHODOLOGY/PRINCIPAL FINDINGS We developed transient and stable transformation systems for Phtheirospermum jap...
متن کاملSmall RNAs Derived from the T-DNA of Agrobacterium rhizogenes in Hairy Roots of Phaseolus vulgaris
Agrobacterium rhizogenes is a pathogenic bacteria that causes hairy root disease by transferring bacterial DNA into the plant genome. It is an essential tool for industry and research due to its capacity to produce genetically modified roots and whole organisms. Here, we identified and characterized small RNAs generated from the transfer DNA (T-DNA) of A. rhizogenes in hairy roots of common bea...
متن کاملIron-binding compounds from Agrobacterium spp.: biological control strain Agrobacterium rhizogenes K84 produces a hydroxamate siderophore.
Iron-binding compounds were produced in various amounts in response to iron starvation by a collection of Agrobacterium strains belonging to the species A. tumefaciens, A. rhizogenes, and A. vitis. The crown gall biocontrol agent A. rhizogenes strain K84 produced a hydroxamate iron chelator in large amounts. Production of this compound, and also of a previously described antibiotic-like substan...
متن کاملUpdate on Genomics of Agrobacterium-Mediated Plant Transformation Agrobacterium in the Genomics Age
Members of the genus Agrobacterium cause the neoplastic diseases crown gall, hairy root, and cane gall on numerous plant species. Extensive genetic analyses conducted in the 1980s identified key bacterial genes involved in virulence. During the past decade, however, genomic technologies have revealed numerous additional bacterial genes that more subtly influence transformation. The results of t...
متن کاملThe Agrobacterium rhizogenes GALLS gene encodes two secreted proteins required for genetic transformation of plants.
Agrobacterium tumefaciens and Agrobacterium rhizogenes are related pathogens that cause crown gall and hairy root diseases, which result from integration and expression of bacterial genes in the plant genome. Single-stranded DNA (T strands) and virulence proteins are translocated into plant cells by a type IV secretion system. VirD2 nicks a specific DNA sequence, attaches to the 5' end, and pil...
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ژورنال
عنوان ژورنال: Molecular Plant-Microbe Interactions®
سال: 2006
ISSN: 0894-0282,1943-7706
DOI: 10.1094/mpmi-19-1385